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Table 1 Intracellular fluxes of the antibody fragment Fab3H6

From: Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure

flux

rate (q)

time constant (K)

half time (t 1/2 )

intracellular protein

formation (Pi)

143.1 μg g-1 h-1 (STD 8.8%)

  

intracellular protein

degradation (Deg)

83.3 μg g-1 h-1 (STD 9.3%)

0.0151 min-1 (STD 2.8%)

45.8 min (STD 2.8%)

protein secretion (Sec)

50.7 μg g-1 h-1 (STD 12.1%)

0.00920 min-1 (STD 2.5%)

75.3 min (STD 2.5%)

dilution by growth (Dil)

9.18 μg g-1 h-1 (STD 9.6%)

0.00167 min-1 (STD 0.05%)

415.9 min (STD 0.05%)

  1. The intracellular fluxes are represented as rates q (μg Fab3H6 (g YDM)-1 h-1), time constants K (min-1) or half times (min). STD = standard deviation; n = 2 independent replicate chemostat cultivations.
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Microbial Cell Factories

ISSN: 1475-2859

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