Figure 4

PNGase F treatment of S. cerevisiae whole cell lysates followed by Western blotting using anti-His antibody. Lysates were prepared from galactose-induced yeast cells, transformed with either an empty vector (lane C) or plasmids expressing the full sequence MeH variant (pFGG3-MeH) and chimeric α-MeH protein with α-factor signal sequence instead of native N-terminal TM domain (pFGG3-alpha-MeH). Lysates with (+) or without (-) PNGase F treatment are indicated below blot lanes. Solid arrows indicate unglycosylated MeH polypeptides, dotted arrows - glycosylated MeH forms.