Skip to main content
Figure 4 | Microbial Cell Factories

Figure 4

From: Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments

Figure 4

Visualization of the increased scFv yield after optimization using SDS-PAGE. Purified TS1-218 expressed under different culture conditions visualized by Coomassie Blue staining. Lane 1: Molecular weight marker PageRuler Plus (Fermentas); Lane 2: Optimized conditions (pH 7.1, 1.2% MeOH, 11°C); Lane 3: Reference conditions (pH 7.5, 1% MeOH, 15.5°C); Lane 4: Pre-optimization conditions (pH 6, 0.5% MeOH, 20°C).

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us