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Figure 2 | Microbial Cell Factories

Figure 2

From: Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli

Figure 2

SDS-PAGE images of total (T), soluble (S), or insoluble (IN) protein fractions of samples from shake flask cultures of E. coli RV308 dsbA+ with the following periplasmic constructs: pCUvar-malE-RI (A, F), pCUvar-ompA-RI (B, G), pCUvar-phoA-RI (C, H) and pCUlac- pelB -RI (D, I) 4 h after induction. Gels represent protein fractions after RI production without DTT (A-E) or with 12 mM DTT (F-J). Gels E and J represent insoluble protein fractions of the constructs without DTT (E) or with 12 mM DTT (J). Numbered lanes: 1 - total protein fraction 10 min before induction, 2 and 3 - soluble and total protein fractions 4 h after induction. Numbered lanes in gels E and J represent the insoluble fractions with the plasmids pCUvar-malE-RI (1A, 1F), pCUvar-ompA-RI (2B, 2G), pCUvar-phoA-RI (3C, 3H) and pCUlac-pelB-RI (4D, 4I). Protein size marker: PageRuler™ Protein Ladder Plus (Fermentas). For growth conditions see Materials and Methods. The amounts of applied protein are normalised.

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Microbial Cell Factories

ISSN: 1475-2859

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