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Archived Comments for: Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli

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  1. Correction of legends of Figs 8 and 9

    Peter Neubauer, Technische Universität Berlin

    19 July 2011

    In this article legend of Figure 8 is mixed up with the legend of Figure 9. The right text is:

    Figure 8: RI activities in the total soluble protein fraction (white bars) and in the periplasmic fraction (grey bars) of E. coli of RV308 pCUlac-pelB-RI and RV308 pCUlac-His6-RI from samples of batch and fed-batch bioreactor cultivations without and with addition of DTT. Data derive from three activity assays.

    Figure 9: The amount of reduced DTT during batch shake flask and fed-batch bioreactor processes with microarerobic production mode, was measured by using Measure-iTTM Kit (Invitrogen). The samples for reduced DTT evaluation were taken every synthesis hour starting with DTT addition moment. The data is derived from three assays.

    Competing interests

    no competing interests.