Figure 3

Segregational stability of pWD and its derivatives in SM20429. Average results of two independent experiments for each construct were presented. Strains harboring the constructs were cultured in the absence of selective pressure, plated under the same conditions, and assayed for plasmid maintenance by replica-plating onto antibiotic-containing media at 40-, 80-, 120-, and 160-generation intervals. The presence of plasmids was finally checked by plasmid preparation and gel electrophoresis.