Table 1 Bacterial strains and plasmids used in this study

Strains

E. coli XL1-Blue

endA1 gyrA96 (nalR) thi-1 recA1 relA1 lac glnV44 F'[::Tn10 proAB+ lacIq Δ(lacZ) M15] hsdR17 (r^K- m^K+)

Stratagene

R. eutropha H16

Wild type (PHB⁺)

DSMZ 428

Plasmids

pBHR68

Amp^r, pBluescript SK^-, harboring PHB synthesis operon from R. eutropha (phaCAB operon), used to produce PHB granules

16

pP-CAB

Amp^r , pBHR68:phaP under control of phaCAB promoter

This study

pFos-P-CAB

Amp^r , pBHR68: bFos-phaP under control of phaCAB promoter

This study

pJun-P-CAB

Amp^r , pBHR68: bJun-phaP under control of phaCAB promoter

This study

pATF2-P-CAB

Amp^r , pBHR68: bATF2-phaP under control of phaCAB promoter

This study

pFos

Amp^r , pBHR68Δ(phaCAB):bFos under control of phaCAB promoter

This study

pFos-P

Amp^r , pBHR68Δ(phaCAB): bFos-phaP under control of phaCAB promoter

This study

pACYC184

Cm^r, Tet^r, cloning vector

Fermentas

pOZ

Cm^r, pACYC184:lacZ under control of phaP promoter

This study

pDBD-Jun-Z

Cm^r, pOZ:DBD-bJun under control of phaCAB promoter

This study

pDBD-Z

Cm^r, pOZ:DBD under control of phaCAB promoter

This study

pPI-LacZ

Amp^r, pTWIN2:phaP-intein-lacZ

17