Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae

Table 4 Content of PPP intermediates in recombinant strains under different acetate condition. ^a

	Metabolite content (nmol/g dry cell weight) ^b
	MT8-1X/TAL		MT8-1X/pGK404
	0 mM Acetate	60 mM Acetate	0 mM Acetate	60 mM Acetate
6PG	18.89 ± 0.58	24.87 ± 1.99	77.71 ± 16.09	33.23 ± 3.77
R5P	14.59 ± 7.64	130.48 ± 10.56	230.12 ± 34.22	409.51 ± 79.03
Ru5P	24.20 ± 5.70	201.70 ± 16.05	217.88 ± 22.98	367.92 ± 72.41
S7P	657.80 ± 8.37	986.61 ± 41.26	3270.01 ± 448.68	6317.95 ± 641.64

^a The values are the averages of three independent experiments, ±SEM.
^b Metabolites were extracted from recombinant S. cerevisiae strains after 24 h fermentation of 40 g/l xylose at 30°C.

ISSN: 1475-2859