Figure 4From: Evolution combined with genomic study elucidates genetic bases of isobutanol tolerance in Escherichia coliacrAB mutations detected in end populations. The acrAB locus was sequenced in clonal isolates from each end population (G1, G2, G3, X1, X2, X3). (A) Discovered mutations mapped onto acrA and acrB protein structures, (B) Mutations in acrA and mexB (an acrB homolog in Pseudomonas aerogenosa) associated with changes in substrate specificity or acrA-mexB assembly.Back to article page