Table 2 Central composite design for the optimization of tryptone, glucose and disodium hydrogen orthophosphate concentrations.
Coded Values* | Real Values* | y= pDNA (mg/L) | ||||||
|---|---|---|---|---|---|---|---|---|
g/L | g/L | g/L | ||||||
Run no. | x 1 | x 2 | x 3 | X 1 | X 2 | X 3 | Actual | Predicted |
1 | -1 | -1 | -1 | 5 | 5 | 5 | 6.99 | 6.75 |
2 | -1 | -1 | 1 | 5 | 5 | 15 | 11.7 | 10.98 |
3 | -1 | 1 | -1 | 5 | 15 | 5 | 6.03 | 6.06 |
4 | -1 | 1 | 1 | 5 | 15 | 15 | 7.95 | 7.74 |
5 | 1 | -1 | -1 | 15 | 5 | 5 | 7.65 | 7.2 |
6 | 1 | -1 | 1 | 15 | 5 | 15 | 12.03 | 11.31 |
7 | 1 | 1 | -1 | 15 | 15 | 5 | 8.43 | 8.46 |
8 | 1 | 1 | 1 | 15 | 15 | 15 | 10.44 | 10.02 |
9 | -1.7 | 0 | 0 | 1.5 | 10 | 10 | 6.27 | 6.6 |
10 | 1.7 | 0 | 0 | 18.5 | 10 | 10 | 8.34 | 8.94 |
11 | 0 | -1.7 | 0 | 10 | 1.5 | 10 | 9.06 | 9.96 |
12 | 0 | 1.7 | 0 | 10 | 18.5 | 10 | 8.25 | 8.28 |
13 | 0 | 0 | -1.7 | 10 | 10 | 1.5 | 6.66 | 6.69 |
14 | 0 | 0 | 1.7 | 10 | 10 | 18.5 | 10.71 | 11.61 |
15 | 0 | 0 | 0 | 10 | 10 | 10 | 11.79 | 11.55 |
16 | 0 | 0 | 0 | 10 | 10 | 10 | 11.43 | 11.55 |