Figure 5

SDS-PAGE analysis of recombinantly produced HRP in fed batch B. Aliquots of the supernatant obtained at different time points during the cultivation of P. pastoris expressing the hrp gene extracellularly at 28°C were loaded onto the gel. lanes 1 and 9, molecular mass standard; lane 3, not induced (after fed-batch phase with glucose); lane 3-8, 7 h; 33 h; 58 h; 76 h; 88 h; 91 h of induction.