Determination of sugar yield in mg of sugars formed/g of wheat straw after 96 h of enzymatic treatment on wheat straw. Saccharification assays were performed in 20 mL of 50 mM sodium phosphate buffer (pH 4.8) containing 5% (w/v) wheat straw. (A) P. cinnabarinus secretome containing CDH or (B) purified rCDH were assayed in the presence of cellulose cocktail at 45°C with an orbital agitation (140 rpm) during 96 h. C: control reaction without CDH. Sugars were quantified by high-performance anion exchange chromatography (HPAEC) coupled with amperometric detection (PAD) equipped with a Carbo-Pac PA-1 analytical column. These experiments were repeated at least three times with similar results. For all-sugars analysis in (A) and (B) statistical significance was P < 0.1 and P < 0.05 respectively.