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Table 3 Measured NADH/NAD ratios and NADH concentration and estimated redox fluxes at steady states of different dilution rates of C.saccharolyticus cultures in the presence and absence of N2 sparging

From: Reassessment of hydrogen tolerance in Caldicellulosiruptor saccharolyticus

Parameter Results obtained with and without N2 sparging at D(h-1) of:
  0.05
(100 mL/min N2)
0.15
(100 mL/min N2)
0.05
no stripping
NADH/NAD (mol/mol) 0.13 ± 0.02 0.10 ± 0.00 0.12 ± 0.00
q NADH produced (mmol/g/h) 4.90 ± 0.2 9.55 ± 0.03 5.8 ± 0.7
q NADH used (mmol/g/h) 0.44 ± 0.03 2.6 ± 0.5 4.0 ± 0.7
q NADH produced/q NADH used (mol/mol) 11 3.7 1.4
NADH available for H2:ase (mmol/g/h) 4.5 7.0 1.8