Table 3 Measured NADH/NAD ratios and NADH concentration and estimated redox fluxes at steady states of different dilution rates of C.saccharolyticus cultures in the presence and absence of N2 sparging
From: Reassessment of hydrogen tolerance in Caldicellulosiruptor saccharolyticus
Parameter | Results obtained with and without N2 sparging at D(h-1) of: | ||
|---|---|---|---|
| Â | 0.05 (100 mL/min N2) | 0.15 (100 mL/min N2) | 0.05 no stripping |
NADH/NAD (mol/mol) | 0.13 ± 0.02 | 0.10 ± 0.00 | 0.12 ± 0.00 |
q NADH produced (mmol/g/h) | 4.90 ± 0.2 | 9.55 ± 0.03 | 5.8 ± 0.7 |
q NADH used (mmol/g/h) | 0.44 ± 0.03 | 2.6 ± 0.5 | 4.0 ± 0.7 |
q NADH produced/q NADH used (mol/mol) | 11 | 3.7 | 1.4 |
NADH available for H2:ase (mmol/g/h) | 4.5 | 7.0 | 1.8 |