Table 2 Enzyme levels (IU.(mg protein-1)) of key catabolic redox-dependent enzymes at steady states at different dilution rates, i.e. GAPDH, ADH, LDH and estimated activity (% of potential activity) of LDH (LDHv) under physiological conditions (Tables 3, 4) based on previously described kinetic model [23], of C. saccharolyticus cultures in the presence and absence of N2 sparging. Presented data is average of one biological and at least three technical replicates at a linear range.
From: Reassessment of hydrogen tolerance in Caldicellulosiruptor saccharolyticus
Enzyme | Results obtained with and without N2 sparging at D(h-1) of: | ||
|---|---|---|---|
| Â | 0.05 (100 mL/min N2) | 0.15 (100 mL/min N2) | 0.05 no sparging |
GAPDH | 3.5 | 1.4 | 1.3 |
ADH | 0.44 | 0.96 | 1.8 |
LDH | 1.2 | 2.6 | 8.3 |
LDHv | 10 | 25 | 100 |