Table 1 Fermentation data in continuous cultivations of C.saccharolyticus on glucose (5g.L-1) at steady states of different dilution rates, with and without N2 sparging.
From: Reassessment of hydrogen tolerance in Caldicellulosiruptor saccharolyticus
Parameter | Results obtained with and without N2 sparging at D(h-1) of: | ||
|---|---|---|---|
| Â | 0.05 (100 mL/min N2) | 0.15 (100 mL/min N2) | 0.05 no stripping |
Biomass conc. (g/L) | 0.51 ± 0.02 | 0.61 ± 0.05 | 0.42 ± 0.01 |
Residual glucose conc. (mM) | 0.05 ± 0.03 | 3 ± 2 | 0.3 ± 0.3 |
q glucose (mmol/g/h) | 2.90 ± 0.09 | 6.2 ± 0.4 | 3.2 ± 0.4 |
q pyruvate (mmol/g/h) | 4.90 ± 0.19 | 9.55 ± 0.03 | 5.8 ± 0.7 |
q H2 (mmol/g/h) | 10. 1 ± 0.4 | 18.0 ± 0.0 | 5.9 ± 0.6 |
Product yield (mol/mol) | Â | Â | Â |
H2 | 3.48 ± 0.09 | 2.9 ± 0.2 | 1.82 ± 0.03 |
Acetate | 1.61 ± 0.03 | 1.32 ± 0.13 | 0.83 ± 0.02 |
Lactate | 0.01 ± 0.00 | 0.01 ± 0.01 | 0.67 ± 0.01 |
Ethanol | 0.07 ± 0.00 | 0.20 ± 0.03 | 0.28 ± 0.04 |
Biomass (g/mol) | 17.3 ± 0.5 | 24.1 ± 1.5 | 16 ± 2 |
YATP (g cells/mol ATP) | 5.4 ± 0.2 | 9.1 ± 0.3 | 6.7 ± 0.6 |
Carbon recovery (%) | 0.96 ± 0.01 | 0.93 ± 0.04 | 0.93 ± 0.02 |
Redox recovery (%) | 0.98 ± 0.01 | 0.93 ± 0.03 | 0.97 ± 0.02 |