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Table 5 Kinetic parameters of Paracoccus sp. 32d β-D-galactosidase

From: A novel cold-active β-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization

Substrate

Temperature (°C)

Km (mM)

kcat (s-1)

kcat/Km (s-1mM-1)

ONPG

10

1.17 ± 0.11

71.81 ± 2.11

61.38

 

20

1.18 ± 0.06

138.3 ± 2.19

117.20

 

30

0.99 ± 0.13

206.00 ± 6.92

208.08

Lactose

10

2.94 ± 0.39

43.23 ± 1.13

15.06

 

20

1.16 ± 0.98

66.67 ± 5.6

57.47

 

30

4.28 ± 0.57

140.00 ± 4.64

32.71

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Microbial Cell Factories

ISSN: 1475-2859

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