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Figure 6 | Microbial Cell Factories

Figure 6

From: High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

Figure 6

Cell lysates on reducing SDS-PAGE gels. A: soluble protein fraction; B: total protein including both soluble and insoluble fractions. The same dilution was used for all samples, i.e. lysates from equal broth volumes were loaded on the gels, and hence the bands represent protein yield per unit volume of culture broth. EB: EnBase; TB: Terrific Broth; ZYM: ZYM-5052 autoinduction medium; UY: Ultra Yield Flask; Erl: Erlenmeyer flask. The values 0.6, 1.5, 3 and 6 associated with EnBase cultures refer to glucose-releasing enzyme concentrations in U l-1.

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Microbial Cell Factories

ISSN: 1475-2859

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