Table 2 Buffers and pH values used for the analysis of thermal stability (Tm) of G. cingulata GDH using Thermo FAD analysis [23].
Buffer | pH | Tm (°C) | pH | Tm (°C) | pH | Tm (°C) |
|---|---|---|---|---|---|---|
Sodium acetate | 4.5 | 55.0 | 5.0 | 56.0 | Â | Â |
Sodium citrate | 4.7 | 55.0 | 5.5 | 54.0 | Â | Â |
Potassium phosphate | 5.0 | 55.5 | 6.0 | 53.5 | 7.0 | 47.5 |
Sodium phosphate | 5.5 | 54.5 | 6.5 | 51.0 | 7.5 | 45.0 |
MES | 5.8 | 56.0 | 6.2 | 54.0 | 6.5 | 53.0 |
HEPES | 7.0 | 50.5 | 8.0 | 43.0 | Â | Â |
Ammonium acetate | 7.3 | 52.5 | Â | Â | Â | Â |
TRIS - HCl | 7.5 | 47.0 | 8.0 | 42.5 | 8.5 | 38.5 |
Imidazole - HCl | 8.0 | 41.5 | Â | Â | Â | Â |
Bicine - HCl | 8.0 | 44.0 | 9.0 | 36.0 | Â | Â |