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Table 2 Buffers and pH values used for the analysis of thermal stability (Tm) of G. cingulata GDH using Thermo FAD analysis [23].

From: Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris

Buffer pH Tm (°C) pH Tm (°C) pH Tm (°C)
Sodium acetate 4.5 55.0 5.0 56.0   
Sodium citrate 4.7 55.0 5.5 54.0   
Potassium phosphate 5.0 55.5 6.0 53.5 7.0 47.5
Sodium phosphate 5.5 54.5 6.5 51.0 7.5 45.0
MES 5.8 56.0 6.2 54.0 6.5 53.0
HEPES 7.0 50.5 8.0 43.0   
Ammonium acetate 7.3 52.5     
TRIS - HCl 7.5 47.0 8.0 42.5 8.5 38.5
Imidazole - HCl 8.0 41.5     
Bicine - HCl 8.0 44.0 9.0 36.0   
  1. The buffers were each 50 mM.