Table 1 Purification of recombinant Glomerella cingulata glucose dehydrogenase.
Purification step | Total activity (U) | Total protein (mg) | Specific activity (U mg-1) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
Clear supernatant | 215,000 | 1,300 | 165 | 100 | 1 |
Phenyl-Sepharose | 160,000 | 192 | 833 | 74 | 5 |
DEAE-Sepharose | 152,000 | 182 | 836 | 71 | 5.1 |