Figure 3

Identification of significant gene expression profiles in relation to the observed IAP expression dynamics. A. The insulin yield on biomass, as a function of supplied phosphate. B. a heat map of the hierarchical clustering analysis based on genes picturing a significant expression profile as a function of supplied phosphate; the lowest examined phosphate level [2.6 mM] was excluded from the SPA analysis; the color code represents the scaled expression values. C.1. Two clusters cA1 (263 genes) and cA2 (202 genes) were selected, harboring genes with declining expression profile with diminishing phosphate levels. The GO slim enrichment analysis identified each four categories, covering 66% (cA1) and 74% (cA2) of genes within each cluster.C.2. Two clusters cB1 (115 genes) and cB2 (78 genes) were selected, harboring genes that showed, similarly to the IAP yield, an increasing expression profile with decreasing phosphate levels. The GO slim enrichment analysis identified three (cB1) and four (cB2) enriched categories, covering 65% (cB1) and 74% (cB2) of gene within each cluster, respectively.