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Table 2 Microfiltration experiment parameters

From: High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production

  MF A MF B MF C
Stirring (rpm) 250-600 250-1000 250-800
Aeration (L·min-1) 1.0-1.4 1.0-1.8 1.0-1.6
pO 2 setting (% DO) 20 20 20-40
Feeding profile MF phase (g·L-1·h-1)    
12-24 hours 2.7→ 4.5 2.7→ 5.4 2.7→ 5.4
24-to the end 4.5→ 3.2 5.4→ 4.6 5.4→ 4.6
Total glycerol fed (g) 236.5 291.8 325.3
Total glycerol consumed (g) 231.9 289.7 320.0
  1. E. coli K4 three different MF experiments: Stirring, aeration and pO2 setting parameters; feeding profiles in MF phase, total glycerol fed and consumed.