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Table 2 Microfiltration experiment parameters

From: High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production

 

MF A

MF B

MF C

Stirring (rpm)

250-600

250-1000

250-800

Aeration (L·min-1)

1.0-1.4

1.0-1.8

1.0-1.6

pO 2 setting (% DO)

20

20

20-40

Feeding profile MF phase (g·L-1·h-1)

   

12-24 hours

2.7→ 4.5

2.7→ 5.4

2.7→ 5.4

24-to the end

4.5→ 3.2

5.4→ 4.6

5.4→ 4.6

Total glycerol fed (g)

236.5

291.8

325.3

Total glycerol consumed (g)

231.9

289.7

320.0

  1. E. coli K4 three different MF experiments: Stirring, aeration and pO2 setting parameters; feeding profiles in MF phase, total glycerol fed and consumed.
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Microbial Cell Factories

ISSN: 1475-2859

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