Figure 7
From: A comparative summary of expression systems for the recombinant production of galactose oxidase
SDS-PAGE analysis and Western blot of the Pichia -codon-optimized GalOx gene with a C-terminal His 6 -tag expressed extracellularly in P. pastoris at 25°C . A. SDS-PAGE analysis of aliquots of the supernatant obtained at different time points during the cultivation of P. pastoris expressing the codon-optimized GalOx gene with a C-terminal His6-tag extracellularly at 25°C; lane 1, molecular mass standard; lane 2-6, 24 h/48 h/72 h/96 h/120 h; B. SDS-PAGE analysis of 10 μL-aliquots of the IMAC purified codon-optimized GalOx gene with a C-terminal His6-tag; lane 1, molecular mass standard; lane 2, 1:10 dilution of GalOx-His6; lane 3, pure GalOx-His6 (2 mg·mL-1); C. Western blot analysis of 10 μL-aliquots of the IMAC purified codon-optimized GalOx gene with a C-terminal His6-tag; lane 1 and 2, Western blot of 1:10 dilution of GalOx-His6 and pure GalOx-His6 (2 mg·mL-1).