Figure 3

De novo secreted IP-10 in the absence of nisin. Three separate cultures (A, B, and C) of NZpSEC:huIP-10 were induced with 10 ng/ml of nisin for 1 hour. Culture A was in the presence of nisin all the time; culture B washed and suspended in fresh medium without inducer at hour 1 and 3, and culture C was washed and suspended in fresh medium without inducer only at hour 1. All cultures were grown a total of 6 hours. Protein extracts were analyzed by Western blot at hour 1, 3, and 6. The band in culture B at hour 6 (asterisk) represents the IP-10 "de novo" specifically secreted from hour 3 to 6 in the total absence of nisin demonstrating the ability of Lactococcus lactis to keep expressing and producing IP-10 for at least three hours.