Figure 1
From: Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by Lactococcus lactis
Schematic design of IP-10 expression system for production and secretion by Lactococcus lactis. The scheme represents the final construction of the IP-10 inducible expression system carried by Lactococcus lactis (pSEC:huIP-10). The diagram shows the nisin-inducible promoter PnisA, the ribosome binding-site of usp45 (RBS), the usp45 signal peptide of the usp45 gene (SPusp), and the coding region for the mature moiety of IP-10. The open circle represents a rho-independent trpA transcription terminator fused just downstream to the IP-10 gene (not to scale).