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Table 1 Plasmids (all integrative) utilized in this study*

From: Lactate production yield from engineered yeasts is dependent from the host background, the lactate dehydrogenase source and the lactate export

Vector

Promoter

Heterologous protein

Selection marker

Reference

pB1

Sc TPI

Bt LDH

URA 3

this study

pB2

Sc TPI

Bt LDH

HIS 3

this study

pB3

Sc TPI

Bt LDH

LEU 2

this study

pBME2

Sc TPI

Bm LDH

URA 3

this study

pBST2

Sc TPI

Bs LDH

URA 3

this study

pLC5

Sc TPI

Lc LDH

URA 3

Brambilla et al., 1999

pLC7

Sc TPI

Lc LDH

HIS 3

Brambilla et al., 1999

p022TLP

Sc TPI

Lp LDH

HIS 3

this study

p012TLP

Sc TPI

Lp LDH

URA 3

this study

p022TLPD94G

Sc TPI

Lp LDHD94G

HIS 3

this study

p012TLPD94G

Sc TPI

Lp LDHD94G

URA 3

this study

p012Jen1

Sc TPI

Sc Jen1

URA 3

this study

p022Jen1

Sc TPI

Sc Jen1

HIS 3

this study

  1. *a complete description of plasmids construction is given in Materials and Methods
  2. Abbreviations: Sc: S. cerevisiae; Bt: Bos taurus; Bm: Bacillus megaterium Bs: Bacillus stearothermophylus; Lc: Lactobacillus casei; Lp: Lactobacillus plantarum