De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial biotechnology
- Jurgen F Nijkamp1, 9,
- Marcel van den Broek2, 9,
- Erwin Datema3, 4, 12,
- Stefan de Kok2, 7, 9,
- Lizanne Bosman2, 9,
- Marijke A Luttik2, 9,
- Pascale Daran-Lapujade2, 9,
- Wanwipa Vongsangnak5, 13,
- Jens Nielsen5,
- Wilbert HM Heijne6,
- Paul Klaassen6,
- Chris J Paddon7,
- Darren Platt7,
- Peter Kötter8,
- Roeland C van Ham3, 4, 12,
- Marcel JT Reinders1, 9, 10,
- Jack T Pronk2, 9,
- Dick de Ridder†1, 9, 10, 11Email author and
- Jean-Marc Daran†2, 9, 11Email author
© Nijkamp et al; licensee BioMed Central Ltd. 2012
Received: 27 January 2012
Accepted: 26 March 2012
Published: 26 March 2012
Saccharomyces cerevisiae CEN.PK 113-7D is widely used for metabolic engineering and systems biology research in industry and academia. We sequenced, assembled, annotated and analyzed its genome. Single-nucleotide variations (SNV), insertions/deletions (indels) and differences in genome organization compared to the reference strain S. cerevisiae S288C were analyzed. In addition to a few large deletions and duplications, nearly 3000 indels were identified in the CEN.PK113-7D genome relative to S288C. These differences were overrepresented in genes whose functions are related to transcriptional regulation and chromatin remodelling. Some of these variations were caused by unstable tandem repeats, suggesting an innate evolvability of the corresponding genes. Besides a previously characterized mutation in adenylate cyclase, the CEN.PK113-7D genome sequence revealed a significant enrichment of non-synonymous mutations in genes encoding for components of the cAMP signalling pathway. Some phenotypic characteristics of the CEN.PK113-7D strains were explained by the presence of additional specific metabolic genes relative to S288C. In particular, the presence of the BIO1 and BIO6 genes correlated with a biotin prototrophy of CEN.PK113-7D. Furthermore, the copy number, chromosomal location and sequences of the MAL loci were resolved. The assembled sequence reveals that CEN.PK113-7D has a mosaic genome that combines characteristics of laboratory strains and wild-industrial strains.
The 1000-dollar genome, an iconic goal in human genomics, is already a reality for the yeast Saccharomyces cerevisiae (based on September 2011 quotes from several sequencing companies for sequencing a 12 Mb genome via paired-end short-read sequencing, at over 40-fold coverage).
Although a high quality reference genome of the laboratory strain S. cerevisiae S288C has been available since 1996 , there are four main reasons to (re)sequence the genomes of other S. cerevisiae strains. First, the considerable sequence divergence among S. cerevisiae species may cause practical complications, for example, the design of oligonucleotide arrays and cassettes for gene disruption in non-S288C strains. The discovery of > 250,000 polymorphisms in 71 S. cerevisiae strains sequenced at low coverage  illustrates that this is not a trivial problem. Secondly, although the genomes of S. cerevisiae strains appears to be much more strongly conserved than those of other organisms, such as E. coli, S. cerevisiae strains do show physiologically relevant differences in their gene complement. For example, the absence of a functional MALx3 gene in S. cerevisiae S288C leads to a maltose-negative phenotype, while an atypical ENA gene complement renders the laboratory strain CEN.PK113-7D more sensitive to lithium ions . The possible importance of strain-specific genes is illustrated by the identification of a probable horizontal gene transfer event in the S. cerevisiae wine strain EC1118, that led to the acquisition of genes from the spoilage yeast Zygosaccharomyces bailii. Third, in addition to the presence or absence of coding regions, differences can occur in non-coding regions, such as promoter regions. Knowledge of such differences is essential for the analysis and modelling of regulatory networks in systems biology . Finally, laboratory evolution is rapidly gaining popularity as a tool to analyse genome function and to select for yeast strains with industrially relevant properties [7–11]. Genome comparisons based on mapping short-read data to a distant relative may overlook structural changes. Hence availability of a well-annotated, high-quality reference genome is essential to interpret the changes that occur during laboratory evolution.
Several wild and domestic yeast strains have been sequenced. At the moment, forty-seven genome projects for S. cerevisiae have been registered at GenBank from which twenty-eight contain a de novo assembled (draft) genome [1, 5, 12–20].
The isogenic family of CEN.PK strains was developed by crossing of different laboratory strains of S. cerevisiae in the 1990's by a consortium of German yeast researchers . A subsequent multi-laboratory study in which four S. cerevisiae strains were compared, confirmed that the CEN.PK strains combine good accessibility to classical and molecular genetics techniques with excellent growth characteristics under controlled, industrially relevant conditions . These strains, and in particular the haploid MATa strain CEN.PK113-7D, have since become extremely popular for studies in systems biology [23, 24]. Moreover, the excellent growth characteristics of the CEN.PK strains have resulted in their broad application in metabolic and evolutionary engineering studies, for example for the fermentation of pentose sugars [25–28], production of ethanol [29, 30] and spirits  production of lactate and pyruvate [32, 33], production of C4-dicarboxylic acids , isoprenoids [35, 36], and fungal polyketide (6-methylsalicylic acid) .
Genomic differences between S. cerevisiae CEN.PK113-7D and the S288C strain have been the subject of several studies. Daran-Lapujade and co-workers  performed a comparative genotyping of the two strains by hybridization of genomic DNA to oligonucleotide gene-expression arrays. This work led to the identification of several genes that were absent in CEN.PK113-7D, but present in S288C. Schacherer and co-workers  employed an oligonucleotide tiling microarray (Affymetrix S. cerevisiae Tiling 1.0R array) based on the S288C genome to detect locations of single nucleotide variation (SNV) in order to narrow down the amount of sequencing needed using traditional sequencing approaches and to find genes absent in CEN.PK113-7D such as RDS1 and EHD3. SNVs in CEN.PK113-7D compared to S288C have previously been characterized by mapping next-generation DNA sequencing data to the S288C reference genome followed by SNV calling . The use of short read (35-bp) sequences and a limited coverage, prohibited detection of insertions and deletions (indels), unique CEN.PK113-7D sequences and structural variations.
The goal of the present study was to make a high-quality assembled and annotated reference genome of S. cerevisiae CEN.PK113-7D sequence available to the academic and industrial yeast research communities. Additionally, we aim to compare the CEN.PK113-7D sequence to that of strain S288C and other previously sequenced S. cerevisiae strains. To this end, we performed high-coverage sequencing, de novo genome assembly, scaffolding and annotation of S. cerevisiae CEN.PK113-7D strain. We explored differences with the S288C genome, including single nucleotide variations, small insertions and deletions (indels) and larger structural variation, copy number variation (CNV) and strain-specific sequences and ORFs.
Results and discussion
Genome assembly, scaffolding and annotation
454 Newbler contigs
Hybrid Mira contigs
Illumina velvet PE scaffolds
Hybrid velvet PE scaffolds
MAIA integrated PE scaffolds
Contigs not in chromosomal Scaffolds
# contigs/scaffolds > 200
16 + 1
Largest contig (kbp)
Total size (Mbp)
Total size contigs > 200 (Mbp)
Tiling on S288C
Coverage on S288C
Reassembly and analysis of replicated sequences
A major challenge of de novo genome assembly with the current read length of 'next generation sequencing' methods is still the identification and reconstruction of repetitive regions. Regions with sequence similarity within the genome are therefore often left unresolved and cause assembly fragmentation. The approximately 38 Long Terminal Repeat (LTR) transposons in the CEN.PK113-7D genome with a length of about 6 kbp each, as well as the whole genome duplication that occurred in the evolutionary history of S. cerevisiae contribute to the presence of repetitive sequences throughout the genome.
S. cerevisiae has five retrotransposon families (Ty1-y5) (Additional file 2: Table S1). Each Ty element is flanked by LTR sequences . A total of 50 LTR flanked retrotransposons have been characterized in the S288C strain , whereas only 17 retrotransposons were identified in the strain YJM789, a strain isolated from the lung of an AIDS patient with pneumonia . Of the 50 retrotransposons found in S288C , evidence of the presence of 40 was obtained in CEN.PK113-7D genome assembly. We found that 39 retrotransposon sequences were indeed located on contig breaks, which suggests an intact transposon or a remnant of one (Additional file 3: Figure S1). A 40th transposon, YCLWTy5-1, was fully de novo assembled. YCLWTy5-1 is the only Ty5-type retrotransposon in S. cerevisiae (SGD). Analysis of the depth-of-coverage ratio between S288C and CEN.PK113-7D gave a rough estimate of the transposon content and suggested that CEN.PK113-7D had 38 Ty retrotransposons, two fewer than the 40 possible retrotransposons derived from the assembly. This difference might be caused by two contig break locations not containing full-length transposons, possibly due to Ty excision by internal recombination, leaving remnant LTRs.
Copy number variation between the S288C and CEN.PK113-7D genomes was estimated by mapping CEN.PK and S288C reads to the S288C genome using BWA 
CNV S288C coordinates
1.01 × 10-100
VII, III, XI
1.63 × 10-21
3.92 × 10-76
6.79 × 10-110
MAL33, MAL31, MAL32, YBR298C-A
2.15 × 10-87
2.81 × 10-138
In contrast to S288C, strains from the CEN.PK lineage are able to grow on maltose as sole carbon source. Both the MAL3 locus and the BIO2/IMA1 locus have double sequence coverage in CEN.PK113-7D (Figure 1A). In S288C the contiguous gene pair BIO2/IMA1 is located upstream of the MAL1 locus on chromosome VII (Figure 1C). As already observed for PHO12 the presence of paralogs complicated the hybridization pattern. IMA1, encoding for an isomaltase, has four paralogs in S288C. Therefore, in addition to the hybridization band on CHRVII corresponding to IMA1, we observed three more hybridized chromosomes, CHRIX (IMA3), CHRX (IMA4, IMA5) and CHRXV (IMA2). In CEN.PK113-7D, the BIO2 and IMA1 hybridization patterns consistently revealed not one but two additional copies of the paired genes on CHRXI and CHRIII. In S288C, BIO2 and IMA1 are physically linked to the locus MAL1 (CHRVII). However, CEN.PK paired-end read mapping to the S288C genome established that the BIO2/IMA1 duplication was located on the right arm subtelomeric region of chromosome III (Figure 1B). Hybridization chromosomal profiles with the MAL32 probes confirmed the co-localization of BIO2/IMA1 and MAL loci on three chromosomes CHRVII, and CHRIII but also on CHRXI as extra copies of BIO2 and IMA1. It was therefore hypothesized that the chromosome III BIO2/IMA1 genes preceded the MAL2 locus as well, given the gene order also observed on the MAL1 locus. This hypothesis was validated by long-range PCR amplification of MAL1, MAL2, and MAL3 in CEN.PK113-7D. The PCR products were sequenced and assembled individually, which was not possible using whole-genome shotgun data because of the high sequence homology. The sequence assembly results confirmed that the additional copy located on chromosome III linked the additional MAL2 locus to BIO2/IMA1. We were not able to reconstruct the MAL4 locus located on CHRXI that seems to share high similarity with CHRIII MAL2.
The maltase MAL22 and maltose permease MAL21 genes are very similar to their MAL3 paralogs, but the regulator gene MAL23 is very different from both the MAL1 and the MAL3 locus. This MAL23 mutant allele also known as MAL2-8 C is responsible for the partial de-repression of the MAL genes in CEN.PK strains in the absence of maltose . Interestingly, the two additional MAL (2 and 4) loci found in CEN.PK113-7D presented features of the two common loci in S288C (MAL1 and MAL3). They probably originated from a two-step event with an initial recombination between MAL1 and 3 at MALx3 genes, placing BIO2 and IMA1 in front of MAL2 or 4, followed by a duplication of newly recombined MAL locus (Figure 1D).
The last region that was called duplicated contained two genes, LEU2 and NFS1. However, this duplication could not be confirmed by Southern blotting of karyotype, nor by direct resequencing of the LEU2 locus.
Single nucleotide variation
Single Nucleotide Variation (SNV) and insertions/deletion (indel) analysis was performed by alignment of the CEN.PK113-7D assembly and the reference sequence of S288C. This analysis identified a total of 21,889 SNVs (Additional file 6: Table S2), of which 13,235 were located in 1,843 open reading frames. 4,677 SNVs (35%) resulted in amino acid changes and affected a total number of 1,406 proteins.
An earlier study by Otero et al (2010), restricting their analysis to genes involved in metabolism, revealed that galactose uptake and ergosterol biosynthesis pathways were enriched for non-synonymous SNVs. In addition to confirming these results, our sequencing data enabled the identification of new variations (Additional file 7: Table S4).
In total (including the mutations found in the PKA pathway) 20 genes encoding for proteins involved in mitogen-activated protein kinase (MAPK) signalling pathway (Additional file 9: Figure S4) showed non-synonymous differences with the S288C sequence. The STE20 gene implicated in pheromone response, pseudohyphal/invasive growth, vacuole inheritance and down-regulation of sterol uptake carried eight such differences. The gene MSN2, involved in the regulation of over 200 genes in response to several stresses, carried one non-synonymous difference .
Small insertions and deletions
In total 2,859 small indels (< 90 bp) were called with an average length of 3 bp. Indels often reside within low-complexity regions, mostly in tandem repeats . 420 indels were found inside a gene, together affecting 297 genes. In 132 genes with indels the start and stop codons usage was not affected (Additional file 10: Table S3).
Interestingly, the indels in SWI1 and SNF11 occur in tandem repeat regions. Such tandem repeats have been shown to predominantly occur in promoter regions and in genes encoding for transcription factors and cell-surface proteins . In S. cerevisiae recombination of intragenic repeats of cell surface proteins has been suggested to be a reversible mechanism of evolutionary adaptation to the environment by creating cell surface diversity . Although 128 genes that encode for transmembrane and cell wall proteins carried at least one indel, this number was not deemed significant (p < 0.05, Bonferroni corrected). However, similarly to analysis of human genome sequences , category enrichment analysis of genes with indels identified overrepresentation of the functional categories 'regulation of transcription' and 'DNA binding' (Figure 3). These repetitive sequences induce contraction and expansion of the gene length by recombination and slippage of the DNA polymerase . In humans, repeat expansion in coding sequences has been implicated in several degenerative diseases as Huntington disease and spinocerebellar ataxias. The genetic cause of Huntington disease is a trinucleotide expansion in exon 1 of the IT15 gene that encodes for huntingtin, a protein involved in several cellular functions including transcription .
The severe effects of tandem repeat length variation in humans raises the question of the effect of this phenomenon in S. cerevisiae. Intriguingly, the physiological implications of these polymorphisms in transcription factors have not yet been studied. Dedicated research in the future should address this to understand the role of such variation.
Absent and specific genes in CEN.PK113-7D relative to the reference S288C
A substantial number of S. cerevisiae genes are redundant as a result of genome duplication, thus allowing the loss of one copy of each duplicated gene. To systematically analyze the genes that were absent in CEN.PK113-7D sequence relative to S288C, a methodical search of S288C homologous genes with at least 95% identity in CEN.PK113-7D was performed. To prevent false negatives (i.e. genes called absent because they were not assembled), genes with a lower copy number (log2 ratio < -0.6) in CEN.PK113-7D than in S288C using the read mapping analysis were listed and compared to those S288C genes that did not present a homolog in CEN.PK113-7D. This analysis identified 83 genes absent in CEN.PK113-7D relative to S288C (Additional file 13: Table S6). Absence of 62 of these genes can be explained by the absence of several large fragments that, in strain S288C, are located in subtelomeric regions (Additional file 13: Table S6), such as on the left arm of CHRI (25 Kbp), on the left arm of CHRVIII (12 kb), on the left arm of CHRXII (17 kb) and on the right arm of CHRXIV (14 kb).
The assembled CEN.PK113-7D genome sequence contained several sequences that are absent in S288C. The CEN.PK specific genes were organized in two large blocks: contig151 and contig596 (Figure 5C). The first block contig151, showed similarity with sequence data of S. cerevisiae strains YJM269 (79%) and PW5 (isolated from Nigerian Raphia palm wine) http://www.genome.wustl.edu/genomes/view/saccharomyces_cerevisiae_pw5 (63%) (Figure 5A). Of the five adjacent genes on contig151, the three new genes could not be functionally annotated. However, their expression could be confirmed by RNA-seq on samples taken from anaerobic carbon- and nitrogen-limited chemostats, commonly used cultivation conditions to study yeast physiology. Interestingly, uncharacterized genes 430, 431 and 432 on contig151 exhibited differential expression and were significantly up-regulated under nitrogen-limitation relative to carbon-limitation [59, 60] (Figure 5C). Furthermore, Southern blotting of chromosomal separation gels established that contig151 could be placed on chromosome XVI (Additional file 14: Figure S3). These observations support the existence and functionality of these open reading frames in CEN.PK113-7D (Figure 5). Functional analysis of these new genes should be part of a larger, emerging challenge: the functional analysis of the growing pangenome of the species S. cerevisiae that arises from the advent of next-generation sequencing.
CEN.PK113-7D specific sequences lead to biotin prototrophy
The CEN.PK113-7D BIO1 and BIO6 did exhibit similarity within the S. cerevisiae pangenome. The genes are conserved (over 99%) in several other S. cerevisiae strains, such as Sigma1278b (laboratory strain used in pseudohyphal studies, GenBank: ACVY00000000.1), YJM269 and UC5 (isolated from Sene sake in Kurashi, Japan, GenBank: AFDD00000000.1) (Figure 5A). Although, the systematic sequence of S288C as reported in SGD indicates that these genes were absent, Hall and Dietrich (2007) identified two pairs of pseudo genes on CHRI ([YAR069W-A (BIO6) and YHR214W-F (BIO8)]) and CHRVIII [YAR070W-A (BIO1) and YHR214W-G (BIO7)] in S288C. Southern blot chromosomal localization of BIO1 and BIO6 genes in S288C and CEN.PK113-7D established that these two genes were indeed exclusively present in CEN.PK113-7D and were located on CHRI. If the pseudogene pairs were indeed present in S288C as previously proposed , they displayed very low degree of similarity. In addition BIO1 and 6 transcripts were detected in glucose- and nitrogen-limited conditions (Figure 5C).
To verify whether these differences in genotype between S288C and CEN.PK113-7D were reflected in phenotype, we tested their growth in chemically defined medium with and without added biotin. After two consecutive shake-flask cultures in biotin-free medium to exhaust biotin reserves, while S288C strain failed to grow (μ < 0.0005 h-1 in 100 hours), the CEN.PK113-7D strain successfully proliferated in the absence of biotin, at a specific growth rate of 0.045 h-1 (compared to 0.38 h-1 in presence of biotin). Biotin is an indispensable water-soluble vitamin that is mainly used as a cofactor by biotin-dependent carboxylases. In the literature, most S. cerevisiae strains are considered to have lost the ability to grow in absence of biotin. To complement this auxotrophy, biotin is routinely included in chemical defined fermentation media. Biotin needs to be dissolved at high pH at which it is not stable. This step is critical in medium preparation and can cause batch-to-batch variation. Strains prototrophic for biotin might offer a solution to this, and CEN.PK113-7D is an excellent model to evaluate the use of biotin prototrophic S. cerevisiae strains for industrial application. Such biotin prototrophy would be economically interesting. A quick estimate based on the amount of biotin used in chemically defined medium  designed to grow around 3 g.l-1 biomass, an average catalog price for biotin of 100 €.g-1 and a fermentation volume of 150 m3 would reduce the medium cost by 1000€. This cost saving might be increased by 5 to 20-fold with media elaborated to grow higher biomass densities (e.g. fed-batch for protein production) .
CEN.PK113-7D shares genomic material with both laboratory and industrial strains
We have sequenced, assembled and annotated the genome of S. cerevisiae CEN.PK113-7D. Complementary to previous re-sequencing efforts , our de novo assembly provides additional insight in still unexplored differences between S. cerevisiae strains, such as large indels. Comparisons of the de novo assembled genome of CEN.PK113-7D to other S. cerevisiae genomes revealed many unstable tandem repeats in genes involved in transcriptional regulation. Repeat expansion and contraction in genes coding for regulatory proteins may be a mechanism of evolving transcription regulation, reconfiguring binding affinity and specificity of transcription factors. From a metabolic engineering perspective, varying tandem repeat lengths may be a promising approach to 'tune' transcription regulation networks.
De novo assembly of the genome allowed us to uncover genes in CEN.PK113-7D that are absent in S288C and relate to phenotypic characteristics specific to CEN.PK113-7D such as maltose metabolism and biotin biosynthesis. These findings led to the surprising discovery that CEN.PK113-7D is biotin prototroph, a phenotypic trait potentially interesting for industrial application. Phylogenetic analysis based on whole-genome sequence established that CEN.PK113-7D is not solely related to laboratory strains like S288C, but also to industrial strains. These genetic specificities are the basis of the robust physiological performance of this strain, which is used as a yeast model for industrial applications. Together, these results contribute to the elucidation of the genetic diversity within the S. cerevisiae pangenome. In addition, a high-quality annotated genome of this popular model strain is now available as a resource to the yeast research community (GenBank BioProject PRJNA52955; http://cenpk.bt.tudelft.nl).
Materials and methods
DNA purification and sequencing
The genomic DNA of S. cerevisiae CEN.PK 113-7D  was purified as previously described . High-throughput sequencing DNA libraries for Illumina (Illumina, San Diego, CA) and 454 (454 Life Sciences, Brandford, CT) sequencing were prepared according the manufacturer's recommendations. The Illumina paired-end library was sequenced on Genome Analyzer IIx (Illumina) with a read length of 50 bp at ServiceXS (Leiden, The Netherlands). The 454 library was sequenced on a GS FLX + system (454 Life Sciences) with an average read length of 350 bp at GATC-Biotech (Konstanz, Germany). The sequence reads were deposited in the Sequence Read Archive (SRA study accession: SRP011487.1).
Assembly, scaffolding, annotation and RNAseq
The two sequencing methods used in this study yielded data with different properties in terms of error models and read length. Different assemblers, i.e. de Bruijn graph and overlap-layout-consensus assemblers, yield optimal results given these data types. We therefore chose a hybrid assembly strategy using the Velvet  assembler for the Illumina data and the Newbler assembler for the 454 data. The output of both assemblers was combined using the Maia algorithm . Paired-end scaffolding was then performed using Velvet's scaffolder . The resulting contigs were placed into chromosomal scaffolds based on homology with the S288C genome (Saccharomyces Genome Database, http://www.yeastgenome.org/, March 3rd, 2011) using MUMmer . The chromosomal scaffolds were generated for visualization purposes. Variation analysis was performed on contigs rather than on scaffolds, as the bear no bias towards S288C.
ORF finding was performed using several tools combined in the Cyrille2 pipeline . Ab initio gene prediction was performed using Augustus , SNAP  and GeneMark-S . Comparative gene prediction was performed by mapping S288C ORFs, S288C proteins and UniProt fungal proteins with GenomeThreader .
The output of the above predictors was combined by Jigsaw  to predict the final ORFs for CEN.PK. These ORFs were aligned using BLAST  to the Saccharomyces Genome Database (SGD) to assign the name of the closest S288C homolog. The assembled data can be retrieved at GenBank [BioProject PRJNA52955] and at http://cenpk.bt.tudelft.nl).
cDNAs from anaerobic carbon-limited, and anaerobic nitrogen-limited chemostat samples  were prepared as previously described . The Illumina cDNA libraries were prepared according the manufacturer's recommendations. The libraries were sequenced on Genome Analyzer IIx with read length of 50 bp at Baseclear (Leiden, The Netherlands). The resulting reads were mapped onto the CEN.PK113-7D genome sequence using the Burrows-Wheeler Alignment tool BWA . Visualization of the mapped reads was performed using the Integrated genomics viewer .
Gap and missing sequence analysis
We investigated the causes of fragmentation and potentially missing sequence between two CEN.PK113-7D contigs. Every CEN.PK113-7D contig was assigned a location in the S288C genome using alignment by MUMmer. Regions in S288C not covered by one of the CEN.PK113-7D contigs, i.e. the missing sequences or gaps, were analyzed. There are several possible causes for missing sequence: (1) the sequence is unique to S288C; (2) the regions were not well amplified in the PCR reaction in the sequencing instrument, because of too high or too low GC content; or (3) the reads are present but not assembled, possibly because of repeats. If the reads are present and the region is not repetitive, a contig break is unlikely. We therefore investigated the latter two causes of gaps: too low read coverage or repeats.
The Illumina reads were mapped to the S288C genome using BWA  and analyzed with Samtools . First we counted the number of regions in the S288C genome between consecutively aligned CEN.PK113-7D contigs that contained at least one base not covered by any read. Such gaps we call missing sequence. Second, we determined if a repetitive sequence could be located in the gap. To this we aligned the S288C genome to itself using MUMmer. Regions were called repetitive if they had > 95% identity with sequence elsewhere in the genome. If a gap overlapped with one of these repetitive regions in S288C, it was labeled unassembled. The total size of missing sequence gaps and unassembled gaps was then tallied, providing insight in the causes of the fragmentation of the assembly.
SNV indels and novel DNA
CEN.PK113-7D contigs were aligned to S288C with MUMmer with an identity threshold of at least 97%. SNVs and indels were called from alignments with a non-ambiguous mapping. Small indels were detected as gapped alignments, maximized to a length of 90 bp by MUMmer's default settings.
We define a region in CEN.PK113-7D to be unique if it shows less than 95% identity to any region in S288C. To list these unique regions, first all non-unique regions were found by whole genome alignment of CEN.PK113-7D and S288C, keeping only alignments with more than 95% identity. For each position in the S288C genome only the best aligned CEN.PK113-7D region was considered; if other regions aligned, this was considered to be a duplication in CEN.PK113-7D, hence unique. All resulting unaligned regions were then called unique to CEN.PK113-7D and analyzed for genes. Conservation of these unique regions in other yeast strains was investigated by aligning them to the NCBI Whole-Genome-Shotgun Sequences database using blastn . Species which contained a similar sequence were selected by sorting the hits on score and subsequently selecting the top hits with query coverage > 60% and identity > 90%.
Enrichment of Gene Ontology (GO) terms in the GO Fat subset  in the sets of SNVs and indels was performed in the DAVID functional annotation tool. P-values were calculated using the EASE score, a modified version of the Fisher exact test . Bonferroni correction was applied by DAVID to account for multiple testing.
Deleted and duplicated genes
Copy number variation of regions in CEN.PK113-7D compared to S288C was investigated by mapping both S288C and CEN.PK113-7D reads to the S288C reference genome. The log2-ratio of the CEN.PK113-7D depth of coverage over that of S288C was calculated using CNV-seq version 0.2-6  and normalized to an average of 1 per chromosome. For this analysis, 36 bp reads for both strains were obtained from . The default CNV-seq threshold of 0.6 was used as a cut-off for the log2-ratio.
S288C genes that could not be mapped to the CEN.PK113-7D assembly by GenomeThreader with an identity score of at least 95% were labeled not present. An additional validation was performed to prevent false positive deletion calls of genes that should be present in the genome but are not assembled; using copy number variation between CEN.PK113-7D and S288C. Genes with a significantly lower copy number value in CEN.PK113-7D were considered not to be present in the data. The intersection of the set of genes not assembled and the set of genes not present in the data resulted in a high confidence list of deleted genes.
Ty retrotransposons mostly cause contig breaks in the assembly process, because their sequences are repetitive in the genome. The presence of the S288C transposons in the CEN.PK113-7D genome can be investigated by using these contig breaks. When inspecting the alignment of CEN.PK113-7D and S288C on locations these Ty elements in the S288C genome, three situations can be observed, the transposon is not present, therefore a gapped alignment of one contig spans the transposon location (GA) with a gap of size similar to the Ty retrotransposon size (~6-kbp); the transposon is present in CEN.PK113-7D, but unassembled, therefore two contigs align to both sides of the transposon location (CB); or the transposon is present in CEN.PK and assembled, therefore a gapless alignment is observed (AS). The first situation yields a not present call for the transposon, the second and third situation yield a present call. The presence of transposons unique to CEN.PK113-7D was not investigated, since this requires long insert mate-pair libraries. An estimate of the number of Ty elements in the CEN.PK113-7D genome for each member of the Ty family (Ty1-Ty5) was obtained by calculating the log2-ratio of the depth of coverage for each Ty element in S288C.
The chromosomes of the CEN.PK113-7D strain were prepared and separated by clamped homogeneous electrical field (CHEF) electrophoresis as previously described in . Transfer of the DNA from the gel to a nylon membrane (Amersham Hybond™-N+, GE Healthcare Europe GmbH, Diegem, Belgium), was performed as previously described in . Genomic DNA probe fragments were amplified from genomic DNA of S. cerevisiae CEN.PK113-7D using Phusion Hot-Start Polymerase (Finnzymes, Landsmeer, The Netherlands) and the oligonucleotides listed in Additional file 5: Table S8. The probes were labelled according to the manufacturer's instructions (Amersham Gene Images AlkPhos Direct Labelling and Detection System, Buckinghamshire, UK). Hybridization was done overnight at 55°C in hybridization buffer (50% formamide, 5× Saline-Sodium Citrate (SSC) buffer, 2% blocking reagent (Roche), 0.1% Na-lauroylsarcosyl, 0.02% Sodium Dodecyl Sulfate (SDS)). Membranes were washed twice with primary wash buffer (2 M urea, 0.1% SDS, 50 mM sodium phosphate, 50 M sodium chloride, 1 mM magnesium chloride, 0.2% blocking reagent) for 15 minutes at 55°C and twice with secondary wash buffer (50 mM Tris base, 0.1 M sodium chloride, 2 mM magnesium chloride) for 5 minutes at room temperature. Digoxigenin-labelled probes were detected by chemiluminescence using CPD-star (Roche, Paris, France).
PCR amplification of the MAL loci
The genomic MAL1, 2 and 3 loci were amplified from genomic DNA of S. cerevisiae CEN.PK113-7D using the Qiagen LongRange PCR kit (Qiagen, Hilden, Germany) according to manufacturer's instructions in a Biometra TGradient Thermocycler (Biometra, Gottingen, Germany). The following primer combinations were used for amplification: ZUO1 Fw/MALx2 Rv (MAL1 locus), YCR102W-A Fw/MALx2 Rv (MAL2 locus) and PHO89 Fw/MALx2 Rv (MAL3 locus) (Additional file 5: Table S8). Sequencing DNA libraries of each of MAL PCR locus for Illumina were prepared and the PCR products were sequenced on Genome Analyzer IIx with read length of 50 bp at Baseclear (Leiden, The Netherlands). The subsequent sequences were assembled using Newbler (454 Life Sciences, Brandford, CT).
Phylogenetic tree construction
The S. cerevisiae genomes were downloaded from GenBank (accession date: 21 Nov. 2011) (Additional file 12: Table S7). Similarities between all pairs of genomes were determined using MUMmer with the settings as recommended in . Pairwise distances were calculated using the coverage distance function . The phylogenetic tree was created by performing hierarchical clustering (UPGMA) with the SplitsTree4 package .
Strains and cultivation conditions
The prototrophic S. cerevisiae strains CEN.PK113-7D  and S288C (ATCC 204508)  were grown in liquid cultures. The shake-flask cultivations with biotin were performed in 500 ml flasks containing 100 ml of medium, which were incubated at 30°C on an orbital shaker set at 200-rpm. The composition of the synthetic medium (SM) was as follows: glucose (20 g.l-1), (NH4)2SO4 (5 g.l-1) KH2PO4 (3 g.l-1), MgSO4 (0.5 g.l-1), trace elements and vitamin solutions . A separate biotin-free vitamin solution was used for growth in absence of biotin. The pH of the medium was adjusted to 5.0 and sterilized by autoclaving. Glucose was autoclaved separately. Vitamins were filter-sterilized and added to the medium. Growth of the various strains was monitored by OD measurements at 660 nm.
We acknowledge the financial support from Netherlands Genomics Initiative (NGI). The PhD project of JFN is funded by the Kluyver Centre for Genomics of Industrial Fermentation. We thank our colleagues of the Industrial Microbiology Section for their support.
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