Microbial Cell Factories

Table 1 Effect of biosynthesis precursors on teicoplanin production.

From: Actinoplanes teichomyceticus ATCC 31121 as a cell factory for producing teicoplanin

		Percentage composition
Addition	Total T-A₂	T-A_2-1	T-A_2-2	T-A_2-3	T-A_2-4	T-A_2-5
None	100	7.3	60.2	13.1	9.1	10.3
Methyl linoleate
0.5 g/L	31	3.2	49.7	16.9	17.1	13.1
1 g/L	28	10.6	49.3	17.0	13.0	10.1
2 g/L	17	29.0	31.0	18.8	10.0	11.2
4 g/L	0	0	0	0	0	0
6 g/L	0	0	0	0	0	0
Trilinoleate
2 g/L	21	21.9	43.1	15.1	10.1	9.8
Methyl oleate
0.5 g/L	38	2.8	56.2	16.3	13.1	11.6
1 g/L	17	2.2	46.2	20.3	19.3	12.0
2 g/L	12	4.0	40.2	25.7	14.9	15.2
4 g/L	10	7.0	31.6	30.7	16.7	14.0
6 g/L	9	5.8	29.9	31.1	17.7	15.5
Trioleate
2 g/L	75	7.6	31.8	49.7	6.1	4.9
L-valine
1 g/L	160	6.2	77	12.9	1, 9.	2
2 g/L	191	6.1	80	11.9	1	1
3 g/L	148	6.3	74	14.2	3	2, 5
L-isoleucine
1 g/L	65	10	42	13	28	7
2 g/L	50	11.1	39.1	12.6	30	7.2
3 g/L	32	10	34.3	14	35.5	6, 2
L-leucine
1 g/L	85	7	50	13	10	23
2 g/L	70	9	49	15	2	25
3 g/L	60	8.1	48	16.6	3.9	23.4

A. teichomyceticus ATCC 31121 was grown in TM1 medium added at the time of inoculum with different biosynthetic precursors. Samples were harvested at 144 hours and analyzed by HPLC as reported in the Methods. Total T-A₂ complex production in control condition (without additions) was set as 100%.

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