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Table 1 Yeasts and plasmids used in this study. Parental strains and their derivatives are shown

From: Molecular and process design for rotavirus-like particle production in Saccharomyces cerevisiae

Strain

Genotype

Plasmid

Plasmid description

Reference

W303-1a

MATa ade2-1 can1-100 ura3-1 leu2-3,112 his3-11,15 trp1-1

-

  

W.TLU-C

 

pRS7, pRS2, pSal4

PPMA1, CEN6/ARSH4, TRP1

PPMA1, 2 μ, LEU2, PCUP1, 2 μ, URA3

This study

W.T-6d

 

pRS7VP6

PPMA1VP6, CEN6/ARSH4, TRP1

This study

CEN.PK.113-5D

MATa SUC2 MAL2-8c ura3-52

-

 

Peter Kottera

CEN.U-C

 

pSP-GM2

PPGK1, PTEF1, 2 μ, URA3

This study

CEN.U-6d

 

pWR6

PPGK1, PTEF1VP6, 2 μ, URA3

This study

CEN-U-267e

 

pWR267

PPGK1ΔVP2, PTEF1VP6, PTEF1VP7 2 μ, URA3

This study

PD 83B.1db

MATa LEU ura31 TRP HIS ADE can1100 GAL SUC2

-

 

Stefan Hohmannc

PD.U-C

 

pSP-GM2

PPGK1, PTEF1, 2 μ, URA3

This study

PD.U-6d

 

pWR6

PPGK1, PTEF1VP6, 2 μ, URA3

This study

PD.U-267e

 

pWR267

PPGK1ΔVP2, PTEF1VP6, PTEF1VP7 2 μ, URA3

This study

PJ69-4a

MATa trp1-901, leu2-3,112, ura3-52, his 3-200, gal4D gal80D

-

  

PJ.U-C

 

pSP-GM2

PPGK1, PTEF1, 2 μ, URA3

This study

PJ.T-6d

 

pRS7VP6

PPMA1VP6, CEN6/ARSH4, TRP1

This study

PJ.U-6d

 

pWR6

PPGK1, PTEF1VP6, 2 μ, URA3

This study

PJ.U-267e

 

pWR267

PPGK1ΔVP2, PTEF1VP6, PTEF1VP7 2 μ, URA3

This study

  1. a. J. W. Goethe Universität, Germany
  2. b. Strain with W303-1a background
  3. c. Gothenburg University, Sweden
  4. d. Contains the VP6 gene.
  5. e. Contains the VP2, VP6 and VP7 genes.