Figure 1From: Molecular and process design for rotavirus-like particle production in Saccharomyces cerevisiaePlasmids constructed in this work. The ΔVP2 gene was cloned under the PGK1 promoter and VP6 and VP7 genes were cloned under individual TEF1 promoters. pWR26 was obtained by cloning the VP6 gene in plasmid pWRΔ2. The cassette TEF1-VP7-CYC1term was amplified by PCR from pWR7 and cloned into pWR26 to obtain pWR267.Back to article page