Fig. 2
Metabolomic revealed the changes in intracellular metabolites induced by ammonium sulfate. a PCA demonstrated intra-group reproducibility and inter-group variability for T1 and T3. b The volcano plot counted and displayed log2FoldChange of the differential metabolites between T3 and T1 and log10P-value, with each point representing a differential metabolite. c Heatmap showed the significantly (P < 0.05) up/down-regulated differential metabolites identified by secondary mass spectrometry. d Match plot showed the significantly up/regulated differential metabolites (VIP-value > 1.5). e KEGG enrichment of differential metabolic pathways. f MS signal intensity of Met and AdoMet in T1 and T3. Notably these values were all significantly greater in T3 than T1. g MS signal intensity of 5mC, 3mA, and 7mG in T1 and T3. h Growth comparison of OE-ThmetH and WT on MM + straw. i Quantification of intracellular Met content and FPA. Intracellular Met content and FPA of OE-ThmetH were significantly higher than that of WT. All results were obtained from hyphae samples grown on T1 and T3, both of which contained 6 biological replicates; red dots resemble values from individual experiments. Student’s t-testing was conducted in (f, g, i), *significant difference to T1 at two-tailed P = 0.022 (g, T3: 5mC), *significant difference to WT at two-tailed P = 0.011 (i, OE-ThmetH: intracellular Met). **significant difference to WT at two-tailed P = 0.004 (i, OE-ThmetH: FPA); ***significant difference to T1 at two-tailed P = 0.000, (f, T3: Met), 0.000 (f, T3: AdoMet), 0.000 (g, T3: 3mA); ns = no statistical difference to T1 at two-tailed P = 0.454 (g, T3: 7mG)