Table 1 Key characteristics of the GFP1-10/GFP11 and GFP1-9/GFP10-11 assembly systems

Basic information

Tag length

16 a.a.

33 a.a.

Pre-maturity

No

Yes

Fluorescence background

Low

High

Supplementary protein preparation

Time required

7 h

5 days

Purification steps

Simple

Complicated

Purification equipment

No

Yes

Incubation parameters

Incubation time

12 h

3 h

Protein added to E. coli (OD₆₀₀ = 1)

15 µM

1 µM

Quantification

Yes

Yes