Fig. 6
From: Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly
Quantitative measurement of surface-displayed laccase using GFP 1–9/GFP10-11 assembly. (a-c) Flow cytometry analyses of OmpA-CotA-GFP10-11, OmpC-CotA-GFP10-11, and InaZ-CotA-GFP10-11 cells with (red) or without (blue) GFP1-9. (d) Whole cell fluorescence intensity of OmpA-CotA-GFP10-11, OmpC-CotA-GFP10-11, and InaZ-CotA-GFP10-11 cells incubated with GFP1-9 protein. (e) Average numbers of CotA-GFP10-11 molecules displayed on OmpA-CotA-GFP10-11, OmpC-CotA-GFP10-11, and InaZ-CotA-GFP10-11 cells. (f) Laccase activity of OmpA-CotA-GFP10-11, OmpC-CotA-GFP10-11, and InaZ-CotA-GFP10-11 cells. The data represent the means ± SD of three independent experiments