Fig. 5
From: Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly
Quantitative measurement of surface-displayed ubiquitin using GFP1-9/GFP10-11 assembly. (a-c) Flow cytometry analysis of OmpA-Ubi-GFP10-11, OmpC-Ubi-GFP10-11, and InaZ-Ubi-GFP10-11 incubated with (red) or without (blue) GFP1-9. The percentages of positive fluorescent cells are indicated. (d) Whole cell fluorescence intensity of OmpA-Ubi-GFP10-11, OmpC-Ubi-GFP10-11, and InaZ-Ubi-GFP10-11 cells incubated with GFP1-9 protein measured using a microplate reader. (e) Standard curve for purified ubiquitin-GFP10-11 and GFP1-9 assembly in-vitro. (f) Average numbers of passenger proteins displayed on OmpA-Ubi-GFP10-11, OmpC-Ubi-GFP10-11, and InaZ-Ubi-GFP10-11 cells. The data represent the means ± SD of three independent experiments