Fig. 4From: Quantitative measurement of cell-surface displayed proteins based on split-GFP assemblyVisualization of surface-displaying cells based on GFP1-9/GFP10-11 assembly. (a) Schematic diagram for the assembly of GFP1-9 and GFP10-11 on the bacterial cell surface; (b) Time-profile of the fluorescence intensity during assembly of GFP1-9 with surface displaying OmpA-Ubi-GFP10-11 cells. (c-e) The overlap of bright field images and corresponding fluorescence images of cells displaying OmpA-Ubi-GFP10-11 (c), OmpC-Ubi-GFP10-11 (d), and InaZ-Ubi-GFP10-11 (e), respectively, incubated with (and without) GFP1-9 proteinBack to article page