Fig. 3
From: Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly
Quantitative measurement of surface-displayed laccase based on GFP1-10/GFP11 assembly. (a-c) Flow cytometry analysis of OmpA-CotA-GFP11, OmpC-CotA-GFP11, and InaZ-CotA-GFP11 incubated with (red) or without (blue) GFP1-10 protein. (d) Whole-cell fluorescence intensity of OmpA-CotA-GFP11, OmpC-CotA-GFP11, InaZ-CotA-GFP11 cells incubated with GFP1-10 protein. (e) Average numbers of CotA-GFP11 proteins displayed on OmpA-CotA-GFP11, OmpC-CotA-GFP11, InaZ-CotA-GFP11 cells. (f) Laccase activity of OmpA-CotA-GFP11, OmpC-CotA-GFP11, and InaZ-CotA-GFP11 cells. The data represent the means ± SD of three independent experiments