Fig. 2
From: Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly
Quantitative assessment of surface-displayed SUMO based on GFP1-10/GFP11 assembly. (a-c) Flow cytometry analysis of OmpA-SUMO-GFP11, OmpC-SUMO-GFP11, InaZ-SUMO-GFP11 cells incubated with (red) or without (blue) GFP1-10 protein. The percentages of positive fluorescent cells are indicated. (d) Whole-cell fluorescence intensity of OmpA-SUMO-GFP11, OmpC-SUMO-GFP11, and InaZ-SUMO-GFP11 cells incubated with GFP1-10 protein measured using a microplate reader. (e) In-vitro standard curve for purified SUMO-GFP11 and GFP1-10 assembly. (f) Average number of passenger proteins displayed on OmpA-SUMO-GFP11, OmpC-SUMO-GFP11 and InaZ-SUMO-GFP11 cells. The data represent the means ± SD of three independent experiments