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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry

Fig. 6

Time series fed-batch flow cytometry bivariate plots. The panels correspond to the time points in Fig. 5A (top row, HaTRPA1) and B (lower row, Δ1-708 HaTRPA1). Note how the cells, most clearly seen in Δ1-708 HaTRPA1, move clockwise from the bottom left quadrant (Q4), through the top left quadrant (Q1) into the top right quadrant (Q2). This means that cells first start to express GFP and HaTRPA1, and then later lose viability. FL1 reports the GFP fluorescence and FL3 the PI staining i.e. damaged cells. The percentage of the total cells is indicated in each quadrant

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Microbial Cell Factories

ISSN: 1475-2859

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