Droplet-based microfluidic platform for detecting agonistic peptides that are self-secreted by yeast expressing a G-protein-coupled receptor

Table 1 Strains, plasmids, and transformants used in this study

Strain, plasmid, or transformant	Relevant features	References
Strain
BY4741	MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0	[34]
IMFD-72ZsD	BY4741 sst2Δ::AUR1-C ste2Δ::LEU2 fig1Δ::ZsGreen his3Δ::P_FIG1-ZsGreen far1Δ gpa1Δ::G_i3tp	[22]
Plasmid
pGK421	Yeast expression vector containing the PGK1 promoter, PGK1 terminator, 2μ origin, and MET15 marker	[35]
pGK421-AGTR1-N295S	Human AGTR1^N295S receptor expression in pGK421	[31]
pGK426	Yeast expression vector comprising the PGK1 promoter, PGK1 terminator, 2μ origin, and URA3 marker	[33]
pGK426-ssAGII	Ang II secretory expression in pGK426	[31]
pGK426-ssAGIII	Ang III secretory expression in pGK426	[31]
pGK426-ssAGIV	Ang IV secretory expression in pGK426	[31]
Transformant
AGTR1-N295S	IMFD-72ZsD / pGK421-AGTR1-N295S	[31]
Mock	AGTR1-N295S / pGK426	[31]
AGII	AGTR1-N295S / pGK426-ssAGII	[31]
AGIII	AGTR1-N295S / pGK426-ssAGIII	[31]
AGIV	AGTR1-N295S / pGK426-ssAGIV	[31]

ISSN: 1475-2859