Fig. 1

Schematic illustration of the engineered yeast and culture method used in this study. A Engineered yeast cells can concurrently express the human AGTR1 receptor (with an N295S mutation) and secrete angiotensin peptides (including analogs). IMFD-72ZsD yeast transformants cointroduced with the GPCR expression plasmid (pGK421-AGTR1-N295S) and the peptide secretion plasmids (pGK426-ssAGII, pGK426-ssAGIII, and pGK426-ssAGIV to secrete the Ang II or its analogs; Ang III and Ang IV) were generated and termed AGII, AGIII, and AGIV, respectively (Table 1). B A microdroplet culture in a W/O emulsion is illustrated as the culture method tested in this study