Table 1 Strains and plasmids used in this research
Strain or plasmid | Description | Source |
|---|---|---|
Strains | ||
E. coli JM109 | The cloning host | This lab |
B. subtilis Q1 | Starting strain, B. subtilis 168 derivative, surfactin high-yielding strain | This lab |
B. subtilis Q1-2 | B. subtilis Q1 derivative, ΔbkdAB | This lab |
Plasmids | ||
pHT01-bkdAA | AmpR(E. coli), ChlR(B. subtilis), P43-bkdAA | This study |
pHT01-bkdAB | AmpR(E. coli), ChlR(B. subtilis), P43-bkdAB | This study |
pHT01-phoD | AmpR(E. coli), ChlR(B. subtilis), P43-phoD | This study |
pHT01-phoB | AmpR(E. coli), ChlR(B. subtilis), P43-phoB | This study |
pHT01-glnA | AmpR(E. coli), ChlR(B. subtilis), P43-glnA | This study |
pHT01-glnJ | AmpR(E. coli), ChlR(B. subtilis), P43-glnJ | This study |
pKS2-bkdAB | KanR(E. coli), KanR(B. subtilis) | This study |