High cell density sequential batch fermentation for enhanced propionic acid production from glucose and glycerol/glucose mixture using Acidipropionibacterium acidipropionici

Table 2 Sequential batch fermentation of glycerol/glucose mixture using A. acidipropionici DSM 4900 with cell recycle

N-source	Batch 1	Batch 2	Batch 3	Batch 4	Batch 5	Batch 6	Batch 7	Batch 8	Batch 9	Batch 10
N-source	HTPJ (1.25 X)						HTPJ (1.25 X) + biotin
Q_p (g/L.h)	0.28	0.36	0.40	0.35	0.33	0.31	0.38	0.39	0.42	0.48
Q_s (g/L.h)	0.59	0.76	0.86	0.70	0.65	0.62	0.76	0.75	0.78	0.93
Y (g/g)	0.48	0.47	0.46	0.50	0.50	0.50	0.50	0.52	0.54	0.51
Y (mol/mol)	0.69	0.71	0.70	0.63	0.75	0.75	0.74	0.78	0.79	0.76
r_P (g_PA/g_X.h)	0.032	0.034	0.032	0.030	0.027	0.024	0.024	0.021	0.021	0.032
Initial CDW (g/L)	0.66	10.28	10.54	12.08	10.98	16.07	12.66	11.13	21.23	21.89
Final CDW (g/L)	8.82	10.65	12.44	11.86	12.44	13.03	15.59	18.30	19.69	21.59
Initial PA (g/L)	0	4.09	4.04	4.00	3.37	3.31	3.53	3.20	4.11	4.40
Final PA (g/L)	32.00	34.98	33.00	36.31	36.10	37.68	37.45	37.80	40.70	40.16
Final AA (g/L)	1.59	1.05	0.74	0.78	0.81	0.95	0.93	2.27	2.54	2.75
Final SA (g/L)	9.76	10.222	10.19	9.58	9.98	10.73	11.37	10.83	10.98	11.01
PA/AA (mol/mol)	16.33	27.01	36.33	37.89	36.17	32.28	32.57	13.51	12.99	11.83
PA:SA:AA (g)	100:31:5	100:29:3	100:31:2	100:26:2	100:28:2	100:28:3	100:30:3	100:29:6	100:27:6	100:27:7

Production of propionic acid from glycerol/glucose mixture using A. acidipropionici DSM 4900 for 10 sequential batches with cell recycling. The compositions of the culture media (heat-treated potato juice and biotin), the concentrations of PA, SA, AA, and biomass, as well as the fermentation kinetics are presented

ISSN: 1475-2859