Fig. 4From: RuBisCO activity assays: a simplified biochemical redox approach for in vitro quantification and an RNA sensor approach for in vivo monitoringIn vivo ADP-specific sensor based fluorescence assay. A The recombinant strains of PCC 6803 (SSL142) were grown with atmospheric CO2 and induced in BG-11 + 50 µg/mL of kanamycin were subjected to the fluorometric assay with different pH (3, 4, 5 or 6) citrate buffers. Their comparative analysis over 150 min indicated a significant increase in the fluorescence at pH 4 followed by 3, 5 and 6. B SSL142 was grown in BG-11 + 50 µg/mL of kanamycin, with and without 20 mM of NaHCO3. Both the cultures were subjected to the fluorescence assay at pH 4. Fluorescence values were normalized with the corresponding OD730, where the results indicated three-fold higher rate of increase in the fluorescence with SSL142 grown in presence of NaHCO3. All the assays were performed in biological triplicatesBack to article page