Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology

Table 1 Quantification of SP4 production by sandwich ELISA

ng SP4 polysaccharide per ul^− 1 of OD₆₀₀ adjusted culture at 5 h
Strain^a	Plasmid			Replicate			Average	Deviation
Strain^a	pB4	pgne	puppS^b	1	2	3	Average	Deviation
W3110	+	-	v	0.60	1.56	0.93	1.03	0.49
W3110	+	-	+	1.58	2.57	1.91	2.02	0.50
CLM37	+	+	v	5.74	6.78	6.72	6.41	0.58
CLM37	+	+	+	24.74	59.46	44.34	42.85	17.41
ΔPGT/GT	+	+	v	10.54	23.06	0.50	11.36	11.30
ΔPGT/GT	+	+	+	47.82	54.83	28.58	43.74	13.60
ng SP4 polysaccharide per ul^− 1of OD₆₀₀adjusted culture at 25 h
W3110	+	-	v	2.80	3.73	3.02	3.18	0.49
W3110	+	-	+	4.19	6.38	4.98	5.18	1.11
CLM37	+	+	v	44.81	70.26	55.40	56.83	12.79
CLM37	+	+	+	63.95	88.22	87.23	79.80	13.74
ΔPGT/GT	+	+	v	62.48	84.84	76.33	74.55	11.28
ΔPGT/GT	+	+	+	79.98	97.34	85.35	87.56	8.89

^aE. coli strains: EJK2 (W3110/pB4/vector), EJK3 (W3110/pB4/puppS), EJK7 (CLM37/pB4/pgne/vector), EJK8 (CLM37/pB4/pgne/puppS), EJK11 (ΔPGT/GT/pB4/pgne/vector), and EJK12 (ΔPGT/GT/pB4/pgne/puppS)
^bPlasmids: v, vector; +, puppS

ISSN: 1475-2859