Fig. 4

Predicted target genes involved in cell growth and morphological differentiation. a EMSA analysis of MtrA binding to promoters of morphological differentiation related genes (lane 1: 50 ng labeled probe, lane 2: 50 ng labeled probe + 3 μg MtrA protein, lane 3: 50 ng labeled probe + 6 μg MtrA protein, lane 4: 50 ng labeled probe + 3 μg MtrA protein + excess unlabeled competing probes). b qRT-PCR validation of day 2 (left) and day 6 (right) fermentation genes bldM, ssgC, whiB and bldD. qRT- PCR used 16S rRNA as a reference gene to obtain the transcript levels of individual genes relative to the reference gene