Primer | Sequence (5’ → 3’) | Function | Refs. |
---|---|---|---|
UP01 | ccgcgctggaggatcatccaTGAGAAGCCTCGCCTTCC | Amplification TS1 fragment, p∆PHB | This work |
UP02 | gccgggtttcGAGAACTGCGTCTTGCGC | Amplification TS1 fragment, p∆PHB | This work |
UP03 | cgcagttctcGAAACCCGGCGCGGCCAA | Amplification TS2 fragment, p∆PHB | This work |
UP04 | tcgttttccgggacgccggcGCCGGCGGGATAGTCGCG | Amplification TS2 fragment, p∆PHB | This work |
UP05 | GCCGGCGTCCCGGAAAAC | Amplification pEMG vector | This work |
UP06 | TGGATGATCCTCCAGCGCG | Amplification pEMG vector | This work |
UP07 | GTGCTGCAAGGCGATTAAGT | Control p∆PHB construct | This work |
UP08 | GCTAAAGCTGGAACGGGGAA | Control p∆PHB construct | This work |
UP09 | GCCCTTCCATGAATTGCGTC | Control p∆PHB construct | This work |
UP10 | GACCACCAAGCGAAACATCG | Control p∆PHB construct | This work |
UP11 | CGGTAGCCGCTTACTTGGC | Control of triparental mating knockout | This work |
UP12 | GCTATGACCATGATTACGCCGG | Control of triparental mating knockout | This work |
UP13 | GACGTTGTAAAACGACGGCC | Control of triparental mating knockout | This work |
UP13 | ACCTCGGCCGCAGTGAT | Control of triparental mating knockout | This work |
UP14 | TCTTCTTGCTAGGCGGGTTG | Verify P. pantotrophus DSM 2944 ∆PHB knockout | This work |
UP15 | GACGATTACGTCAGCGCCTA | Verify P. pantotrophus DSM 2944 ∆PHB knockout | This work |
8F | AGAGTTTGATCCTGGCTCAG | 16S Fwd | [83] |
1492R | CGGTTACCTTGTTACGACTT | 16S Rev | [84] |