A genetic toolbox to empower Paracoccus pantotrophus DSM 2944 as a metabolically versatile SynBio chassis

Table 2 Efficiency of transformation.

	Antibiotic marker	Conc. (mg/L)	Genebank seq	Transformation efficiency (cfu/µg)		Conjugation
	Antibiotic marker	Conc. (mg/L)	Genebank seq	Electroporation	Heat shock	Without helper strain	With helper strain
pSEVA121	Ampicillin	100	JX560322	No colonies	No colonies	No colonies	No colonies
pSEVA221	Kanamycin	50	JX560327	2 × 10⁴	No colonies	No colonies	2 × 10⁶
pSEVA321	Chloramphenicol	10	JX560332	No colonies	No colonies	No colonies	1 × 10¹
pSEVA421	Spectinomycin	100	JX560337	2 × 10²	No colonies	2 × 10⁶	2 × 10⁸
pSEVA521	Tetracycline	20	JX560342	No colonies	No colonies	No colonies	No colonies

This table highlights the heat-shock and electroporation-based transformation and conjugation results of P. pantotrophus with five different SEVA plasmids. Italics underlined highlights positive and italics represnts negative transformation and conjugation results, as described by the number of colony-forming units (cfu) per microgram (µg) of added plasmid

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