Fig. 3

Kinetic comparison in SDS-PAGE (12%) of total protein (TP) produced in cultures of E. coli BL21 (DE3) grow up in shake flasks (A, B) and bioreactors (C, D). RBD-P producing cultures are shown in (A) and (C) and RBD in (B) and (D). In all gels, lanes 1 and 6 correspond to PT before induction. Lanes 2, 3, and 4 (7,8, and 9) show samples taken from cultures at 1, 5, and 10 h after induction, respectively. Lane 5 corresponds to the molecular weight marker (MWM). Lanes 6 to 9 are samples from replica experiments. Recombinant proteins are marked by a black arrow