Fig. 1
From: Quantifying microbial robustness in dynamic environments using microfluidic single-cell cultivation
Dynamic microfluidic single-cell cultivation. Photos of (a) the PDMS-based dynamic microfluidic cultivation chip, (b) the microfluidic structure and (c) a single oscillation-structure. Each structure contains six arrays of 23 cultivation chambers in the oscillation region and one separate array to the right that acted as control for the starvation condition. (d) Feast medium, containing glucose (blue), and starvation medium (red) are introduced via inlets. The left cultivation structure is constantly perfused with feast medium. (e) The flow profile in the oscillation structures can be switched between feast and starvation. (f) In each chamber, yeast cells grew in a monolayer under constant perfusion with either feast or starvation medium that was switched every 1.5 min