Strain or plasmid | Relevant characteristicsa | Origin |
---|---|---|
Strain | ||
E. coli DH5α | F– φ80lacZ∆M15 ∆(lacZYA-argF) U169 deoR recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Novagen |
E. coli 0K | E. coli W3110 (DE3) | [27] |
E. coli BT-1 | E. coli 0K containing plasmid pETPtac-xylBC | This study |
E. coli 1K | E. coli W3110 (DE3)ΔxylA | [27] |
E. coli BT-2 | E. coli 1K containing plasmid pETPtac-xylBC | This study |
E. coli 3K | E. coli 1KΔyjhHΔyagE | [27] |
E. coli BT-3 | E. coli 3K containing plasmid pETPtac-xylBC | This study |
E. coli 4K | E. coli 3KΔxynR | [27] |
E. coli BT-4 | E. coli 4K containing plasmid pETPtac-xylBC | This study |
E. coli 4KI | E. coli W3110 (DE3)ΔxylAΔyjhHΔyagEΔxynR with PT7-xylD-kdcA expression cassette knocking in the position of xynR in chromosome | [27] |
E. coli BT-5 | E. coli 4KI containing plasmid pETPtac-xylBC | This study |
E. coli 5KI | E. coli 4KIΔptsG | This study |
E. coli BT-6 | E. coli 5KI containing plasmid pETPtac-xylBC | This study |
E. coli BT-7 | E. coli 5KI with PT7-xylBC knocking in the position of ptsG in chromosome | This study |
E. coli BT-8 | E. coli 5KI with PT7-xylBC knocking in the position of mgsA in chromosome | This study |
E. coli BT-9 | E. coli 5KI with xylBC knocking in the position of xylA in chromosome | This study |
E. coli BT-10 | E. coli BT-9Δpgi | This study |
Plasmid | ||
pETPtac-xylBC | Expression vector, pETPtac with gene xylBC | [7] |
pTKRED | Plasmid expressing Red recombinase genes; Sper | Addgene |
pCP20 | Plasmid expressing Flp recombinase to remove kan cassette; Cmr | CGSCb |
pKD4 | Template for kanamycin resistance cassette; Kanr | CGSCb |