Table 1 Strains and plasmids used in this study
Plasmid/strain | Relevant characteristics | Reference |
|---|---|---|
Plasmids | ||
pYB1k | p15A ori, pBAD promoter, KanR | Laboratory |
pLB1s | R6K ori, pBAD promoter, StrR | Laboratory |
pRB1k | RSF1030, pBAD promoter, KanR | Laboratory |
pXB1k | p15A ori, pBAD promoter, KanR | Laboratory |
pLB1k | R6K ori, pBAD promoter, KanR | Laboratory |
pSB1k | pSC101 ori, pBAD promoter, KanR | Laboratory |
P01 | pYB1k containing HpFucT-EcGmd-EcWcaG genes | Laboratory |
P02 | pLB1s containing EcManC-EcManB genes | Laboratory |
P03 | pYB1k containing EcGmd-EcWcaG-HpFucT genes | Laboratory |
P04 | pYB1k containing HpFucT-EcManC-EcManB genes | This study |
P05 | pLB1s containing EcGmd-EcWcaG genes | This study |
P06 | pYB1k containing EcManC-EcManB-HpFucT genes | This study |
P07 | pRB1k containing HpFucT-EcManC-EcManB genes | This study |
P08 | pXB1k containing HpFucT-EcManC-EcManB genes | This study |
P09 | pXB1k containing HpFucT-EcManC-EcManB-EcGmd-EcWcaG genes | This study |
P10 | pXB1k containing HpFucT-EcGmd-EcWcaG-EcManC-EcManB genes | This study |
P11 | pXB1k containing HpFucT-EcManC-EcGmd-EcWcaG-EcManB genes | This study |
P12 | pXB1k containing HpFucT-EcGmd-EcManC-EcManB-EcWcaG genes | This study |
P13 | pLB1k containing HpFucT-EcGmd-EcWcaG-EcManC-EcManB genes | This study |
P14 | pSB1k containing HpFucT-EcGmd-EcWcaG-EcManC-EcManB genes | This study |
P15 | pXB1k containing HpFucT gene | This study |
P16 | pXB1k containing HpFucT-EcManA genes | This study |
P17 | pXB1k containing HpFucT-EcManB genes | This study |
P18 | pXB1k containing HpFucT-EcManC genes | This study |
P19 | pXB1k containing HpFucT-EcGmd genes | This study |
P20 | pXB1k containing HpFucT-EcWcaG genes | This study |
P21 | pXB1k containing HpFucT-EcGmd-EcWcaG-EcManC-EcManB-RcsAB genes | This study |
P22 | pXB1k containing HpFucT-RcsAB genes | This study |
P23 | pXB1k containing AsFucT-EcGmd-EcWcaG-EcManC-EcManB-RcsAB genes | This study |
P24 | pXB1k containing SAMT-EcGmd-EcWcaG-EcManC-EcManB-RcsAB genes | This study |
P25 | pXB1k containing DeFucT-EcGmd-EcWcaG-EcManC-EcManB-RcsAB genes | This study |
P26 | pXB1k containing MuFucT-EcGmd-EcWcaG-EcManC-EcManB-RcsAB genes | This study |
P27 | pXB1k containing EcWbgL-EcGmd-EcWcaG-EcManC-EcManB-RcsAB genes | This study |
P28 | pXB1k containing PsFucT-EcGmd-EcWcaG-EcManC-EcManB-RcsAB genes | This study |
P29 | pXB1k containing CaFucT-EcGmd-EcWcaG-EcManC-EcManB-RcsAB genes | This study |
P30 | pXB1k containing Hp11FucT-EcGmd-EcWcaG-EcManC-EcManB-RcsAB genes | This study |
P31 | pXB1k containing BKHT-EcGmd-EcWcaG-EcManC-EcManB-rcsAB genes | This study |
Strains | ||
E. coli trans T1 | Wild-type | Invitrogen |
E. coli BW25113 | lacIqrrnBT14∆lacZWJ16hsdR514∆araBADAH33∆rhaBADLD78 | Invitrogen |
SG104 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs | Laboratory |
S01 | SG104,ΔpfkA,ΔpfkB | This study |
S02 | SG104,ΔpfkA,ΔpfkB,Δpgi | This study |
S03 | SG104,ΔpfkA,ΔpfkB,Δpgi,119-lacY | This study |
S04 | S03,ΔwcaF::119-gmd-wcaG-manC-manB | This study |
S05 | S04,119-gapA | This study |
S06 | S04,119-AGG-gapA | This study |
S07 | S04,119-AGGAGG-gapA | This study |
S08 | S04,119-AGGAGGAGG-gapA | This study |
S09 | S04,119-AGGAGGAGGAGG-gapA | This study |
RFL01 | SG104 harboring plasmids P01 and P02 | This study |
RFL02 | S01 harboring plasmids P01 and P02 | This study |
RFL03 | S02 harboring plasmids P01 and P02 | This study |
RFL04 | S03 harboring plasmids P01 and P02 | This study |
RFL05 | S03 harboring plasmids P03 and P02 | This study |
RFL06 | S03 harboring plasmids P04 and P05 | This study |
RFL07 | S03 harboring plasmids P06 and P05 | This study |
RFL08 | S03 harboring plasmids P07 and P05 | This study |
RFL09 | S03 harboring plasmids P08 and P05 | This study |
RFL10 | S03 harboring plasmid P09 | This study |
RFL11 | S03 harboring plasmid P10 | This study |
RFL12 | S03 harboring plasmid P11 | This study |
RFL13 | S03 harboring plasmid P12 | This study |
RFL14 | S03 harboring plasmids P13 | This study |
RFL15 | S03 harboring plasmids P14 | This study |
RFL16 | S03 harboring plasmid P15 | This study |
RFL17 | S04 harboring plasmid P10 | This study |
RFL18 | S04 harboring plasmid P15 | This study |
RFL19 | S04 harboring plasmid P16 | This study |
RFL20 | S04 harboring plasmid P17 | This study |
RFL21 | S04 harboring plasmid P18 | This study |
RFL22 | S04 harboring plasmid P19 | This study |
RFL23 | S04 harboring plasmid P20 | This study |
RFL24 | S04 harboring plasmid P21 | This study |
RFL25 | S04 harboring plasmid P22 | This study |
RFL26 | S04 harboring plasmid P23 | This study |
RFL27 | S04 harboring plasmid P24 | This study |
RFL28 | S04 harboring plasmid P25 | This study |
RFL29 | S04 harboring plasmid P26 | This study |
RFL30 | S04 harboring plasmid P27 | This study |
RFL31 | S04 harboring plasmid P28 | This study |
RFL32 | S04 harboring plasmid P29 | This study |
RFL33 | S04 harboring plasmid P30 | This study |
RFL34 | S04 harboring plasmid P31 | This study |
RFL35 | S05 harboring plasmid P30 | This study |
RFL36 | S06 harboring plasmid P30 | This study |
RFL37 | S07 harboring plasmid P30 | This study |
RFL38 | S08 harboring plasmid P30 | This study |
RFL39 | S09 harboring plasmid P30 | This study |